Introduction
Enzyme Linked ImmunoSorbent Assay (ELISA) is one of the most common immunodetection technologies, which is widely used in the diagnosis and prognosis monitoring of cancer, infection, autoimmunity and other diseases. Based on the specific binding of antigen and antibody, this method can quantitatively determine the content of index substances in biological samples such as plasma, serum, urine, interstitial fluid, etc. ELISA is widely accepted in clinic with advantages of high sensitivity, good specificity, convenient operation and low cost of use.
The development of ELISA diagnostic kits involves developing antigen and antibody reagents, establishing immunodetection methods, production methods and kits’ performance evaluation. HKIG has many years of experience in the field of in vitro diagnostic product development, and has helped partners develop a number of innovative ELISA kits, which are successfully registered as medical device.
HKIG provides customers with a comprehensive “one-stop” ELISA diagnostic kit development solution, providing customers with all technical support in the ELISA diagnostic kit development process.
Example
A cytokine is an important effector molecule of inflammatory response and a pathogenic factor of many autoimmune diseases. Using the EFFIMABTM precision monoclonal antibody development platform, HKIG has developed a series of high-affinity monoclonal antibodies that bind to the human and mouse cytokines for customers. HKIG has further screened and obtained a paired monoclonal antibody that recognizes the cytokine with high affinity, using which constructed a high-sensitivity ELISA immunoassay kit. The above work has provided a solid foundation for the client to carry out the development of diagnostic products targeting this cytokine.
Sandwich ELISA to detect the target cytokine. LoB<0.5ng/mL R2>0.99